Development of nineteen Taqman real-time PCR assays for screening and detection of select highly pathogenic bacteria
نویسندگان
چکیده
منابع مشابه
Development of TaqMan Duplex Real-time PCR for Simultaneous Detection of Chlamydia Trachomatis and Mycoplasma Genitalium
Background and Objective: Sexually infections transmitted by bacteria are one of thetherapeutic and social problemsworldwide. The Real-time PCR assay is one of the most sensitive diagnostic and screening methods for these infections. The purpose of this study wassimultaneous detection of Chlamydia trachomatis and Mycoplasma genitaliumusing the TaqMan duplex real-time polymerase chain reaction. ...
متن کاملDevelopment of TaqMan-based real-time PCR assays for diagnostic detection of Babesia bovis and Babesia bigemina.
Two TaqMan-based real-time polymerase chain reaction (PCR) assays devised for the detection of two bovine Babesia parasites, Babesia bovis and B. bigemina, were evaluated for their diagnostic utility using cultured parasites and 92 field bovine blood samples collected from cattle living in Brazil. The real-time PCR assays were compared with previously established nested-PCR assays. The detectio...
متن کاملraPid deteCtion of total and PatHogeniC VIBRIo pARAHAemoLyTICus Using real-time PCr witH taqman® flUoresCent Probes
Vibrio parahaemolyticus is found throughout the marine environment and is a major cause of foodborne illness around the world. Foodborne illnesses due to V. parahaemolyticus infections frequently trace back to the consumption of raw or undercooked seafood. The ability to detect V. parahaemolyticus in a rapid, sensi tive, and specific manner is important to safeguard our food supply and prevent...
متن کاملRapid multiplex PCR and real-time TaqMan PCR assays for detection of Salmonella enterica and the highly virulent serovars Choleraesuis and Paratyphi C.
Salmonella enterica is a human pathogen with over 2,500 serovars characterized. S. enterica serovars Choleraesuis and Paratyphi C are two globally distributed serovars. We have developed a rapid molecular-typing method to detect serovars Choleraesuis and Paratyphi C in food samples by using a comparative-genomics approach to identify regions unique to each serovar from the sequenced genomes. A ...
متن کاملDevelopment of one-step TaqMan® real-time reverse transcription-PCR and conventional reverse transcription-PCR assays for the detection of equine rhinitis A and B viruses
BACKGROUND Equine rhinitis viruses A and B (ERAV and ERBV) are common equine respiratory viruses belonging to the family Picornaviridae. Sero-surveillance studies have shown that these two viral infections are prevalent in many countries. Currently, the diagnosis of ERAV and ERBV infections in horses is mainly based on virus isolation (VI). However, the sensitivity of VI testing varies between ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Infection Ecology & Epidemiology
سال: 2018
ISSN: 2000-8686
DOI: 10.1080/20008686.2018.1553462